Identify your audience. To create an effective bio you need to determine who will be reading your bio and what impression you want them to have. The purpose of your bio will help identify the audience. For a job application the audience will be employers, for a speaking engagement the audience will be comprised of people interested in the topic you are speaking on.
Take a step back to reflect. You're chronicling your life lessons, but what have you learned from them? Relay your intentions, desires, feelings of loss, feelings of joy, the wisdom you've gained, and other inner thoughts from time to time throughout the book. Taking a step back from the action of the story to reflect on what it all means is a good way to add depth to your autobiography.
Develop a central theme, main idea or thesis that clearly explains the purpose behind your autobiography. For example, your theme might be about how you overcame obstacles to become a stronger person, or it might explain why a particular passion -- such as mountain climbing or rescuing animals -- is so important to you. Back your theme with details and background information about your life experiences. For example, you might explain that your dad is in the military, so you've had to learn how to adapt quickly to new schools and unfamiliar environments. Or, you might discuss what it was like growing up in a poor, single-parent home, but how your family was always willing to take in injured or stray pets.