Weave in themes. Use the major themes of your life as a way to tie stories together, connecting your past and your present. Aside from the central conflict, what themes have followed you throughout your life? A fondness for certain holidays, your fascination with a certain place that you visited over and over, a certain type of guy you've always fallen for, a rich spiritual life you fall back on again and again. Bring up the themes every so often to help form a cohesive picture of your life.
Identify your audience. To create an effective bio you need to determine who will be reading your bio and what impression you want them to have. The purpose of your bio will help identify the audience. For a job application the audience will be employers, for a speaking engagement the audience will be comprised of people interested in the topic you are speaking on.
Create an overarching plot. Now that you know what content you want to include in your autobiography, think about how you want to structure your book. Like any great book, your autobiography needs a great plot. Work with the material you have to craft an interesting story that builds toward a climax and ultimately resolves. Create a narrative arc by organizing and filling out your written memories and anecdotes so that they flow together logically.
Develop a central theme, main idea or thesis that clearly explains the purpose behind your autobiography. For example, your theme might be about how you overcame obstacles to become a stronger person, or it might explain why a particular passion -- such as mountain climbing or rescuing animals -- is so important to you. Back your theme with details and background information about your life experiences. For example, you might explain that your dad is in the military, so you've had to learn how to adapt quickly to new schools and unfamiliar environments. Or, you might discuss what it was like growing up in a poor, single-parent home, but how your family was always willing to take in injured or stray pets.